Lab Services is de officiële BNL distributeur voor Biospec beads

Ook voor Biospec beads bent u bij Lab Services aan het juiste adres. De meeste beads hebben we standaard op voorraad en zijn hierdoor snel leverbaar. Voor meer informatie of een prijsopgave verzoeken wij u om het contactformulier in te vullen. For Cell Disruption we suggest: 

Size

  • When working with Bacteria use the 0.1mm diameter glass beads.
  • When working with Yeast/Fungi use the 0.5mm diameter glass beads.
  • When working with Most Tissue use the 1.0mm diameter glass beads or zirconia/silica beads.
  • When working with Skin or “soft” plant material use a 2.0mm diameter zirconia beads.
  • When working with really tough or fibrous tissue use the same sized beads (see above) but choose a more dense bead material. For example, researchers prefer 0.1mm zirconia-silica beads for disruption of spores or 2.3 mm chrome-steel beads for extraction of tough fibrous plant material like monocot leaves. Some users have had good results using the MiniBeadbeater in a “dry grinding” process at liquid nitrogen temperatures.
Density

  • Glass has a density of 2.5 g/cc (most commonly used bead media for “Beadbeating”)
  • Zirconia/Silica has a density of 3.7g/cc (50% more dense than glass – good for spores and most tissues)
  • Silicon Carbide (sharp particle, not a bead) has a density of 3.2 g/cc (May work faster on tissue samples because the particles have sharp cutting edges. Their utility is still under investigation, but see Brein’s comments below)
  • Zirconia has a density of 5.5g/cc (100% more dense than glass – good for tough tissue)
  • Steel, stainless or not, has a density of 7.9g/cc (used mostly for grinding leaves – stainless steel beads are expensive and but can be reused. Chrome steel beads are 10X cheaper – cheap enough to be use one time and thrown away.)
  • Tungsten Carbide has a density of 14.9g/cc (While very dense, this bead is not recommended for biopreps – leaves prep dirty)
Other uses for beads:

  • Quick and easy plating of yeast and bacteria. Add a few sterile 6.3mm diameter glass beads to the plate to evenly spread yeast and bacteria inoculums. No need to remove them afterward.
  • Pack roller bottles, tubes or vials with 6.3 mm diameter glass beads to greatly increase the surface area for tissue culture growth. In this application the beads should be packed tightly to prevent movement of the beads during rolling or shaking. When growing cell in non-agitated culture flasks, confluence is reached faster and cell density is enhanced by adding a layer of 0.1 mm diameter beads (see Growth of Three Established Cell Lines on Glass Microcarriers by James Carani, et. al., Biotechnology and Bioengineering, Vol.25, p.1359-1372 (1983).
  • Create a bio-reactor by packing a column with glass beads and inoculate with select surface-adhering micro-organisms or cells.
  • Keep dialysis tubes vertical during dialysis by adding a few large beads before sealing
Beads (Guide-Lines)
CLICK HERE for detailed information on:

  • Selecting the correct beads for cell disruption
  • Bead Properties
  • Other applications for beads
  • Cleaning beads
  • Useful user commentary
  • Ways to load beads in vials
  • and more
Product specifications
  • Glass beads
  • Glass has a density of 2.5 g/cc. Beads are sold in one pound bottles.

    Cat. No. / Product Description
    11079101/ 0.1 mm diameter
    11079105 / 0.5 mm diameter
    11079110 / 1.0 mm diameter
    11079125 / 2.5 mm diameter
    11079135 / 3.5 mm diameter
    11079635 / 6.4 mm diameter
  • Zirkonia/Silica beads
  • Zirconia/Silica has a density of 3.7g/cc (50% more dense than glass - good for spores and most tissues) Beads are sold in one pound bottles.

    Cat. No. / Product Description
    11079101Z / 0.1 mm diameter
    11079105Z / 0.5 mm diameter
    11079110Z / 1.0 mm diameter
    11079125Z / 2.3 mm diameter
  • Zirkonia beads
  • Zirconia has a density of 5.5g/cc (100% more dense than glass - good for tough tissue) Beads are sold in one pound bottles.

    Cat. No. / Product Description
    11079107ZX / 0.7 mm diameter
    11079110ZX / 1.0 mm diameter
    11079124ZX / 2.0 mm diameter
  • Stainless Steel beads
  • Stainless steel has a density of 7.9g/cc (used mostly for grinding leaves). Note: These beads are not sold in one pound amounts.

    Cat. No. / Product Description / Ordering Amount
    11079114SS / 1.4 mm diameter / 45 gr
    11079123SS / 2.3 mm diameter / 90 gr
    11079132SS / 3.2 mm diameter / 90 gr
    11079635SS / 6.35 mm diameter / 100 beads
  • Chrome Steel beads
  • Chrome steel has a density of 7.9g/cc (used mostly for grinding leaves). Beads are sold in one pound bottles.

    Cat. No. / Product Description
    11079113C / 1.3 mm diameter
    11079123C / 2.3 mm diameter
    11079132C / 3.2 mm diameter
    11079635C / 6.35 mm diameter
  • Silicon Carbide
  • Silicon Carbide (sharp particle, not a bead) has a density of 3.2 g/cc. Particles are sold in a one pound bottle.
    Preliminary information suggests that plant and animal tissue can be disrupted more quickly with sharp-edged material rather than smooth beads.
    Note: The sharp, hard edges of these particles impart knife-like cutting action during high-energy shaking that may abrade and micro-chop tough tissue samples faster than beads. Wash with lab detergent before first use.

    Cat. No. / Product Description
    11079101SC / 0.1 mm diameter
    11079110SC / 1.0 mm diameter
    11079140SC / 4.0 mm diameter
  • Tungsten Carbide beads
  • Tungsten Carbide has a density of 14.9g/cc. While very dense, this bead is not recommended for biopreps. It will leave your prep dirty.
    Note: These beads impart a brown-gray color to homogenates. This inert, colloidal tungsten-carbide can often be removed by centrifugation or filtration.

    Cat. No. / Product Description / Ordering Amount
    11079111WC / 0.1 mm diameter / 90 gr
Neem contact met ons op voor meer informatie
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