Automated extraction and qPCR setup

Introduction

At the center of most molecular laboratories you will find DNA or RNA extractions followed by numerous PCR workflows, be it qPCR, conventional PCR or SNP marker analyses. Manual pipetting and manual extractions are prone to user errors and provide less repeatability over multiple experiments. Automation of these steps will provide better accuracy and repeatability.

While carrying out manual extractions laboratory technicians can come into contact with hazardous chemicals or with hazardous samples posing serious health risks. Using an automated platform to standardize extraction methods helps to increase precision, accuracy, robustness and better repeatability of the extraction method and other manual labor steps.

Application purpose

This application is created for the user to have a perfect walk away solution for extracting DNA or RNA followed by downstream processes requiring a PCR step. The design of this system will provide an easy and accessible solution to provide the user with both a robust extraction method and a PCR workflow. The main advantage that this system will offer is minimal hands-on time which will free up the user’s time to focus on other activities. As laboratory space is a premium we have designed this setup in a way to have the smallest custom made footprint as possible. It features several state of the art liquid handling platforms as well as a fully automated qPCR instrument. Fully controlled by our innovative PlateButler^® software.

Why you should choose this solution

This solution provides a fast and reproducible workflow for both sample extraction and various PCR workflows. DNA and RNA extraction as well as PCR workflows are one of the main applications for any molecular biology laboratory. Its small footprint allows for a spot in almost every environment.